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Real-time PCR (RT-PCR) is a technology that allows to quantitate the number of specific DNA or RNA molecules in studied samples. It is a sensitive (up to single molecule detection) and robust (linear in wide range of concentrations) method. It requires specific instrument, which costs about 50 000 EUR. One experiment (100-400 samples in parallel) takes about half a day to perform.


Real-time polymerase chain reaction (Wikipedia)
Real-time PCR tutorial



Source: www.mosbio.eu