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Quantitative mass spectroscopy (MS) is a technology that allows to quantitate the number of specific protein molecules in studied samples.

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Real-time PCR (RT-PCR) is a technology that allows to quantitate the number of specific DNA or RNA molecules in studied samples. It is a sensitive (up to single molecule detection) and robust (linear in wide range of concentrations) method. It requires specific instrument, which costs about 50 000 EUR. One experiment (100-400 samples in parallel) takes about half a day to perform.

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Two-dimensional electrophoresis is a well known method for protein separation which is extremely useful in the field of proteomics. The basic idea is to separate proteins contained in a sample using two independent properties such isoelectric point and mass. In the gel, each spot represents a protein accumulation and its size depends on the amount of protein present in the sample.

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The quantitative immunoblot is a similar approach to ELISA. It uses the same principles as the ELISA, a reliance on the highly specific antibody antigen interaction to measure the amount of a given protein in a complex mixture. The major distinguishing feature of this method is the inclusion of a step to electrophoretically separate the proteins in the sample according to their molecular weights. Thus, all samples can be solubilized using a standardized procedure and analyzed concurrently for a number of interesting proteins.

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Reporter genes are genes that will be attached to a specific gene under investigation. It is used to verify if a certain gene was taken up by an organism of interest or if it was expressed in a cell. Reporter genes are easier detectable through the characteristics they confer to the host organism. Typical reporter genes are those that encode fluorescent and luminescent proteins like GFP (green fluorescent protein). These proteins are visually detectable. A reporter gene is usually transplanted together with the gene of interest in a so called DNA construct (generally a plasmid) into the cell.